We work in human renal proximal tubular HK-2 cells (PTC) and in prostate epithelial cancer cells (non-cancerous and cancerous cell lines). In PTC, we analyse the role of intracellular prostaglandin E2 (iPGE2) in the damage and repair of the proximal renal tubule, with the aim of developing new therapeutic intervention routes based on the modulation of iPGE levels or the activation of intracellular PGE receptors (iEP). In prostate cells, we analyze the role of iPGE2 in the exacerbation of neoplasm-related phenotypes. In both cases, we pay particular attention to the regulation of HIF-1α by iPGE2 and to the role of HIF-1α as a mediator of the effects of iPGE2.
Madrigal-Martínez A, Fernández-Martínez AB, Lucio Cazaña FJ. Intracrine prostaglandin E2 pro-tumoral actions in prostate epithelial cells originate from non-canonical pathways.J Cell Physiol 2018; 233:3590-3602
Fernandez-Martínez AB, Benito Martínez S, Lucio-Cazaña FJ Intracellular prostaglandin E2 mediates cisplatin-induced proximal tubular cell death. Biochim Biophys Acta (Molecular cell Research) 2016; 1863:293-302
Martínez AB, Lucio-Cazaña FJ Transactivation of EGFR by prostaglandin E2 receptors: a nuclear story? Cell Mol Life Sci 2015; 72:2187-2198
Fernández-Martínez AB, Arenas-Jimenez MI, Lucio-Cazaña FJ Retinoic acid increases hypoxia-inducible factor-1 through intracrine prostaglandin E2 signalling in human renal proximal tubular cells HK-2 Biochem Biophys Acta [Molecular and Cell Biology of Lipids] 2012; 1821:672-683.
Fernández-Martínez AB, Arenas-Jiménez MI, Moreno-Manzano V, Lucio-Cazaña FJ Intracrine prostaglandin E2 signalling regulates hypoxia-inducible factor-1α expression through retinoic acid receptor-ß Biochem Cell Biol. 2014; 44:2185-2193